cd19 beads Search Results


anti-cd19 magnetic beads immunoprecipitation (ip) kit  (Sino Biological)
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This antibody was obtained from a rabbit immunized with purified, recombinant Human CD19 (rh CD19; Catalog#11880-H08H; P15391; Met1-Lys291).
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anti-cd19 magnetic beads  (Nexcelom Bioscience)
90
Nexcelom Bioscience anti-cd19 magnetic beads
Deletion of Tnfsf11 from osteocytes prevents ovariectomy-induced bone loss. 6-Month-old female Tnfsf11f/f and Tnfsf11ΔOt mice were either sham-operated (white bars) or ovariectomized (OVX) (dark gray bars) and killed 6 weeks later. A, uterine weight (n = 10–12 animals per group). B, real time PCR quantification of Tnfsf11 genomic DNA in femoral cortical bone, <t>CD19+</t> bone marrow cells, CD19− bone marrow cells, spleen, kidney, liver, and muscle (n = 3–12). C, μCT images of vertebral cancellous bone and femoral cortical bone. Bar = 500 μm. D, bone volume over tissue volume (BV/TV) of cancellous bone measured in lumbar vertebra by μCT (n = 10–12). E, cortical thickness of the femoral midshaft measured by μCT (n = 10–12). F and G, osteoclast surface per bone perimeter (Oc.S/B.Pm) and osteoclast number per bone perimeter (Oc.N/B.Pm) measured by histomorphometric analysis of lumbar vertebra (n = 6–10). H and I, real time RT-PCR of Tnfsf11 and Tnfrsf11b mRNA in tibial cortical bone (n = 10–12). J, Tnfsf11 mRNA expression in CD19+ bone marrow cells (n = 3–5). *, p < 0.05.
Anti Cd19 Magnetic Beads, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd19+beads/anti+cd19+magnetic+beads/pmc05122756-78-7-17
Average 90 stars, based on 1 article reviews
anti-cd19 magnetic beads - by Bioz Stars, 2026-09
90/100 stars
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cd19 protein coupled magnetic beads  (ACROBiosystems)
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Image Search Results


Deletion of Tnfsf11 from osteocytes prevents ovariectomy-induced bone loss. 6-Month-old female Tnfsf11f/f and Tnfsf11ΔOt mice were either sham-operated (white bars) or ovariectomized (OVX) (dark gray bars) and killed 6 weeks later. A, uterine weight (n = 10–12 animals per group). B, real time PCR quantification of Tnfsf11 genomic DNA in femoral cortical bone, CD19+ bone marrow cells, CD19− bone marrow cells, spleen, kidney, liver, and muscle (n = 3–12). C, μCT images of vertebral cancellous bone and femoral cortical bone. Bar = 500 μm. D, bone volume over tissue volume (BV/TV) of cancellous bone measured in lumbar vertebra by μCT (n = 10–12). E, cortical thickness of the femoral midshaft measured by μCT (n = 10–12). F and G, osteoclast surface per bone perimeter (Oc.S/B.Pm) and osteoclast number per bone perimeter (Oc.N/B.Pm) measured by histomorphometric analysis of lumbar vertebra (n = 6–10). H and I, real time RT-PCR of Tnfsf11 and Tnfrsf11b mRNA in tibial cortical bone (n = 10–12). J, Tnfsf11 mRNA expression in CD19+ bone marrow cells (n = 3–5). *, p < 0.05.

Journal: The Journal of Biological Chemistry

Article Title: RANKL (Receptor Activator of NFκB Ligand) Produced by Osteocytes Is Required for the Increase in B Cells and Bone Loss Caused by Estrogen Deficiency in Mice *

doi: 10.1074/jbc.M116.742452

Figure Lengend Snippet: Deletion of Tnfsf11 from osteocytes prevents ovariectomy-induced bone loss. 6-Month-old female Tnfsf11f/f and Tnfsf11ΔOt mice were either sham-operated (white bars) or ovariectomized (OVX) (dark gray bars) and killed 6 weeks later. A, uterine weight (n = 10–12 animals per group). B, real time PCR quantification of Tnfsf11 genomic DNA in femoral cortical bone, CD19+ bone marrow cells, CD19− bone marrow cells, spleen, kidney, liver, and muscle (n = 3–12). C, μCT images of vertebral cancellous bone and femoral cortical bone. Bar = 500 μm. D, bone volume over tissue volume (BV/TV) of cancellous bone measured in lumbar vertebra by μCT (n = 10–12). E, cortical thickness of the femoral midshaft measured by μCT (n = 10–12). F and G, osteoclast surface per bone perimeter (Oc.S/B.Pm) and osteoclast number per bone perimeter (Oc.N/B.Pm) measured by histomorphometric analysis of lumbar vertebra (n = 6–10). H and I, real time RT-PCR of Tnfsf11 and Tnfrsf11b mRNA in tibial cortical bone (n = 10–12). J, Tnfsf11 mRNA expression in CD19+ bone marrow cells (n = 3–5). *, p < 0.05.

Article Snippet: E , bone marrow cells isolated using anti-CD19 magnetic beads were counted using a cellometer Auto 2000 (Nexcelom Bioscience) ( n = 3).

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing

Deletion of Tnfsf11 from osteocytes prevents the increase in bone marrow B cells after ovariectomy. A–D, percentage of B cells, T cells, monocytes, and erythrocyte progenitors in bone marrow analyzed by flow cytometry (n = 5–6). OVX, ovariectomized. E, bone marrow cells isolated using anti-CD19 magnetic beads were counted using a cellometer Auto 2000 (Nexcelom Bioscience) (n = 3). F, percentage of B cells, T cells, monocytes, and erythrocytes in bone marrow of Tnfsf11f/f and Tnfsf11ΔOt mice by flow cytometry (n = 8). G and H, real time RT-PCR of IL-7 and CXCL12 mRNA in lumbar vertebra (n = 9–11). *, p < 0.05.

Journal: The Journal of Biological Chemistry

Article Title: RANKL (Receptor Activator of NFκB Ligand) Produced by Osteocytes Is Required for the Increase in B Cells and Bone Loss Caused by Estrogen Deficiency in Mice *

doi: 10.1074/jbc.M116.742452

Figure Lengend Snippet: Deletion of Tnfsf11 from osteocytes prevents the increase in bone marrow B cells after ovariectomy. A–D, percentage of B cells, T cells, monocytes, and erythrocyte progenitors in bone marrow analyzed by flow cytometry (n = 5–6). OVX, ovariectomized. E, bone marrow cells isolated using anti-CD19 magnetic beads were counted using a cellometer Auto 2000 (Nexcelom Bioscience) (n = 3). F, percentage of B cells, T cells, monocytes, and erythrocytes in bone marrow of Tnfsf11f/f and Tnfsf11ΔOt mice by flow cytometry (n = 8). G and H, real time RT-PCR of IL-7 and CXCL12 mRNA in lumbar vertebra (n = 9–11). *, p < 0.05.

Article Snippet: E , bone marrow cells isolated using anti-CD19 magnetic beads were counted using a cellometer Auto 2000 (Nexcelom Bioscience) ( n = 3).

Techniques: Flow Cytometry, Isolation, Magnetic Beads, Quantitative RT-PCR

Deletion of Esr1 from B cells does not alter bone mass or B cell number. 6-Month-old female Esr1f/f and Esr1ΔB mice were either sham-operated or ovariectomized (OVX) and killed 6 weeks later. A, real time PCR quantification of Esr1 genomic DNA in CD19+ cells, CD19− cells, muscle, kidney, and liver (n = 3–8). B, real time RT-PCR of Esr1 and Esr2 mRNA in ovary and CD19+ cells in Esr1f/f and Esr1ΔB mice (n = 6–11). N.D., not detected. C, uterine weight (n = 9–13 animals per group). D, μCT images of vertebral cancellous bone and femoral cortical bone. Bar = 500 μm. E, bone volume over tissue volume (BV/TV) of cancellous bone measured in lumbar vertebra by μCT (n = 9–13). F, cortical thickness of the femoral midshaft measured by μCT (n = 9–13). G–K, percentage of B cell populations in bone marrow measured by flow cytometry: total B cells (B220+/CD19+), pro-B cells (B220lo/CD43+/CD19+/IgM−), pre-B cells (B220 lo/CD43−/CD19+/IgM−), immature B cells (B220 lo/CD43−/CD19+/IgM+), mature B cells (B220 hi/CD43−/CD19+/IgM+) (n = 5–8). *, p < 0.05.

Journal: The Journal of Biological Chemistry

Article Title: RANKL (Receptor Activator of NFκB Ligand) Produced by Osteocytes Is Required for the Increase in B Cells and Bone Loss Caused by Estrogen Deficiency in Mice *

doi: 10.1074/jbc.M116.742452

Figure Lengend Snippet: Deletion of Esr1 from B cells does not alter bone mass or B cell number. 6-Month-old female Esr1f/f and Esr1ΔB mice were either sham-operated or ovariectomized (OVX) and killed 6 weeks later. A, real time PCR quantification of Esr1 genomic DNA in CD19+ cells, CD19− cells, muscle, kidney, and liver (n = 3–8). B, real time RT-PCR of Esr1 and Esr2 mRNA in ovary and CD19+ cells in Esr1f/f and Esr1ΔB mice (n = 6–11). N.D., not detected. C, uterine weight (n = 9–13 animals per group). D, μCT images of vertebral cancellous bone and femoral cortical bone. Bar = 500 μm. E, bone volume over tissue volume (BV/TV) of cancellous bone measured in lumbar vertebra by μCT (n = 9–13). F, cortical thickness of the femoral midshaft measured by μCT (n = 9–13). G–K, percentage of B cell populations in bone marrow measured by flow cytometry: total B cells (B220+/CD19+), pro-B cells (B220lo/CD43+/CD19+/IgM−), pre-B cells (B220 lo/CD43−/CD19+/IgM−), immature B cells (B220 lo/CD43−/CD19+/IgM+), mature B cells (B220 hi/CD43−/CD19+/IgM+) (n = 5–8). *, p < 0.05.

Article Snippet: E , bone marrow cells isolated using anti-CD19 magnetic beads were counted using a cellometer Auto 2000 (Nexcelom Bioscience) ( n = 3).

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Flow Cytometry

Osteoclastogenic potential of B cell preparations. A, diagram showing method to culture osteoclasts from isolated bone marrow cells. 5 × 105 CD19+ cells and 1.5 × 104 CD19− cells were plated per well of 48-well plates and cultured for 5 or 8 days. B, flow cytometric analysis of cells isolated by magnetic beads. C, TRAP staining of osteoclasts from CD19+ and CD19− cells after incubation with M-CSF and RANKL. Left, low magnification; scale bar, 200 μm. Right, high magnification; scale bar, 50 μm.

Journal: The Journal of Biological Chemistry

Article Title: RANKL (Receptor Activator of NFκB Ligand) Produced by Osteocytes Is Required for the Increase in B Cells and Bone Loss Caused by Estrogen Deficiency in Mice *

doi: 10.1074/jbc.M116.742452

Figure Lengend Snippet: Osteoclastogenic potential of B cell preparations. A, diagram showing method to culture osteoclasts from isolated bone marrow cells. 5 × 105 CD19+ cells and 1.5 × 104 CD19− cells were plated per well of 48-well plates and cultured for 5 or 8 days. B, flow cytometric analysis of cells isolated by magnetic beads. C, TRAP staining of osteoclasts from CD19+ and CD19− cells after incubation with M-CSF and RANKL. Left, low magnification; scale bar, 200 μm. Right, high magnification; scale bar, 50 μm.

Article Snippet: E , bone marrow cells isolated using anti-CD19 magnetic beads were counted using a cellometer Auto 2000 (Nexcelom Bioscience) ( n = 3).

Techniques: Isolation, Cell Culture, Magnetic Beads, Staining, Incubation

CD19-Cre does not label osteoclasts in vitro. A, images of tdTomato fluorescence in CD19+ cells immediately after isolation from mice of the genotypes indicated at the left. tdTomato fluorescence is in red, 20× magnification. B, fluorescent and brightfield images of osteoclasts in cultures of CD19+ cells from mice of the indicated genotypes after 8 days of culture. tdTomato fluorescence is in red, DAPI nuclear staining is in blue, and TRAP staining in the brightfield image is in red. Scale bar, 20 μm. C, fluorescence and brightfield images of osteoclast cultures containing different ratios of BMM from LysM-Cre;tdTomato and tdTomato mice. The ratio of LysM-Cre;tdTomato to tdTomato cells decreased from left to right. The top panels are fluorescent images with tdTomato fluorescence in red and DAPI fluorescence in blue. The bottom panels are brightfield images showing TRAP staining in red. Scale bar, 50 μm.

Journal: The Journal of Biological Chemistry

Article Title: RANKL (Receptor Activator of NFκB Ligand) Produced by Osteocytes Is Required for the Increase in B Cells and Bone Loss Caused by Estrogen Deficiency in Mice *

doi: 10.1074/jbc.M116.742452

Figure Lengend Snippet: CD19-Cre does not label osteoclasts in vitro. A, images of tdTomato fluorescence in CD19+ cells immediately after isolation from mice of the genotypes indicated at the left. tdTomato fluorescence is in red, 20× magnification. B, fluorescent and brightfield images of osteoclasts in cultures of CD19+ cells from mice of the indicated genotypes after 8 days of culture. tdTomato fluorescence is in red, DAPI nuclear staining is in blue, and TRAP staining in the brightfield image is in red. Scale bar, 20 μm. C, fluorescence and brightfield images of osteoclast cultures containing different ratios of BMM from LysM-Cre;tdTomato and tdTomato mice. The ratio of LysM-Cre;tdTomato to tdTomato cells decreased from left to right. The top panels are fluorescent images with tdTomato fluorescence in red and DAPI fluorescence in blue. The bottom panels are brightfield images showing TRAP staining in red. Scale bar, 50 μm.

Article Snippet: E , bone marrow cells isolated using anti-CD19 magnetic beads were counted using a cellometer Auto 2000 (Nexcelom Bioscience) ( n = 3).

Techniques: In Vitro, Fluorescence, Isolation, Staining

CD19-Cre does not label osteoclasts in vivo. Shown are fluorescence and bright field microscopy images of histological frozen sections of lumbar vertebra from LysM-Cre;tdTomato mice, tdTomato mice, and CD19-Cre;tdTomato mice. Images on the left are fluorescence showing tdTomato-positive cells in bone and bone marrow. Images on the right are brightfield images of the same sections after staining for TRAP activity. Scale bar, 20 μm.

Journal: The Journal of Biological Chemistry

Article Title: RANKL (Receptor Activator of NFκB Ligand) Produced by Osteocytes Is Required for the Increase in B Cells and Bone Loss Caused by Estrogen Deficiency in Mice *

doi: 10.1074/jbc.M116.742452

Figure Lengend Snippet: CD19-Cre does not label osteoclasts in vivo. Shown are fluorescence and bright field microscopy images of histological frozen sections of lumbar vertebra from LysM-Cre;tdTomato mice, tdTomato mice, and CD19-Cre;tdTomato mice. Images on the left are fluorescence showing tdTomato-positive cells in bone and bone marrow. Images on the right are brightfield images of the same sections after staining for TRAP activity. Scale bar, 20 μm.

Article Snippet: E , bone marrow cells isolated using anti-CD19 magnetic beads were counted using a cellometer Auto 2000 (Nexcelom Bioscience) ( n = 3).

Techniques: In Vivo, Fluorescence, Microscopy, Staining, Activity Assay